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human si gr construct  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology human si gr construct
    Human Si Gr Construct, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gr+sirna/pmc11932930-236-9-12?v=Santa+Cruz+Biotechnology
    Average 92 stars, based on 28 article reviews
    human si gr construct - by Bioz Stars, 2026-07
    92/100 stars

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    Knockdown of KLF15 and FoxO3a diminished protective effects of <t>HMPA.</t> <t>Myotubes</t> were transfected with GR, FoxO3a and NT <t>siRNA</t> as described in the Materials and Methods section, followed by the analysis of (A) GR, (B) KLF15 and (C) FoxO3a mRNA expression. The results are expressed as means ± SEM ( n = 4 per group). * p <0.05, ** p <0.01, *** p <0.001 indicates the significant difference between indicated groups. (D, E) Ubiquitin ligases Atrogin-1 and MuRF-1 expression in NT, GR/KLF15 and FoxO3a-siRNA transfected myotubes. Transfected myotubes were treated with HMPA for 1 ‍h as pretreatment followed by Dex for 24 ‍h. The results are expressed as means ± SEM ( n = 4 per group). * p <0.05, ** p <0.01, *** p <0.001 indicates the significant difference between indicated groups. siRNA, small interfering non-targeting RNA; GR, Glucocorticoid receptor; NT, non-targeting.
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    human si gr construct  (Santa Cruz Biotechnology)
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    Knockdown of KLF15 and FoxO3a diminished protective effects of <t>HMPA.</t> <t>Myotubes</t> were transfected with GR, FoxO3a and NT <t>siRNA</t> as described in the Materials and Methods section, followed by the analysis of (A) GR, (B) KLF15 and (C) FoxO3a mRNA expression. The results are expressed as means ± SEM ( n = 4 per group). * p <0.05, ** p <0.01, *** p <0.001 indicates the significant difference between indicated groups. (D, E) Ubiquitin ligases Atrogin-1 and MuRF-1 expression in NT, GR/KLF15 and FoxO3a-siRNA transfected myotubes. Transfected myotubes were treated with HMPA for 1 ‍h as pretreatment followed by Dex for 24 ‍h. The results are expressed as means ± SEM ( n = 4 per group). * p <0.05, ** p <0.01, *** p <0.001 indicates the significant difference between indicated groups. siRNA, small interfering non-targeting RNA; GR, Glucocorticoid receptor; NT, non-targeting.
    Human Si Gr Construct, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gr+sirna/pmc11932930-236-9-12?v=Santa+Cruz+Biotechnology
    Average 92 stars, based on 1 article reviews
    human si gr construct - by Bioz Stars, 2026-07
    92/100 stars
    		  Buy from Supplier

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    Knockdown of KLF15 and FoxO3a diminished protective effects of HMPA. Myotubes were transfected with GR, FoxO3a and NT siRNA as described in the Materials and Methods section, followed by the analysis of (A) GR, (B) KLF15 and (C) FoxO3a mRNA expression. The results are expressed as means ± SEM ( n = 4 per group). * p <0.05, ** p <0.01, *** p <0.001 indicates the significant difference between indicated groups. (D, E) Ubiquitin ligases Atrogin-1 and MuRF-1 expression in NT, GR/KLF15 and FoxO3a-siRNA transfected myotubes. Transfected myotubes were treated with HMPA for 1 ‍h as pretreatment followed by Dex for 24 ‍h. The results are expressed as means ± SEM ( n = 4 per group). * p <0.05, ** p <0.01, *** p <0.001 indicates the significant difference between indicated groups. siRNA, small interfering non-targeting RNA; GR, Glucocorticoid receptor; NT, non-targeting.

    Journal: Journal of Clinical Biochemistry and Nutrition

    Article Title: 3-(4-Hydroxy-3-methoxyphenyl) propionic acid mitigates dexamethasone-induced muscle atrophy by attenuating Atrogin-1 and MuRF-1 expression in mouse C2C12 skeletal myotubes

    doi: 10.3164/jcbn.23-70

    Figure Lengend Snippet: Knockdown of KLF15 and FoxO3a diminished protective effects of HMPA. Myotubes were transfected with GR, FoxO3a and NT siRNA as described in the Materials and Methods section, followed by the analysis of (A) GR, (B) KLF15 and (C) FoxO3a mRNA expression. The results are expressed as means ± SEM ( n = 4 per group). * p <0.05, ** p <0.01, *** p <0.001 indicates the significant difference between indicated groups. (D, E) Ubiquitin ligases Atrogin-1 and MuRF-1 expression in NT, GR/KLF15 and FoxO3a-siRNA transfected myotubes. Transfected myotubes were treated with HMPA for 1 ‍h as pretreatment followed by Dex for 24 ‍h. The results are expressed as means ± SEM ( n = 4 per group). * p <0.05, ** p <0.01, *** p <0.001 indicates the significant difference between indicated groups. siRNA, small interfering non-targeting RNA; GR, Glucocorticoid receptor; NT, non-targeting.

    Article Snippet: Myotubes were transfected with the GR siRNA (Thermo Fischer Scientific), FoxO3a siRNA (Silencer ® Select siRNA FoxO3; ID - s80658) or non-targeting (NT) siRNA (Thermo Fischer Scientific) using the transfection reagent lipofectamine RNAiMAX (Thermo Fisher Scientific).

    Techniques: Knockdown, Transfection, Expressing